全文获取类型
收费全文 | 146篇 |
免费 | 7篇 |
出版年
2023年 | 1篇 |
2021年 | 6篇 |
2020年 | 5篇 |
2019年 | 2篇 |
2018年 | 1篇 |
2017年 | 4篇 |
2016年 | 9篇 |
2015年 | 9篇 |
2014年 | 6篇 |
2013年 | 14篇 |
2012年 | 14篇 |
2011年 | 10篇 |
2010年 | 7篇 |
2009年 | 3篇 |
2008年 | 6篇 |
2007年 | 7篇 |
2006年 | 6篇 |
2005年 | 1篇 |
2004年 | 4篇 |
2003年 | 2篇 |
2002年 | 2篇 |
2001年 | 2篇 |
2000年 | 3篇 |
1999年 | 2篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 3篇 |
1991年 | 5篇 |
1990年 | 2篇 |
1988年 | 4篇 |
1982年 | 1篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1977年 | 2篇 |
1975年 | 1篇 |
1972年 | 2篇 |
排序方式: 共有153条查询结果,搜索用时 0 毫秒
151.
Muntjeeb M. Syed Dilip D. Dhavale Jignesh B. Doshi Sudam L. Kate Girish Kulkarni 《Journal of biomolecular structure & dynamics》2020,38(9):2717-2736
AbstractSickle cell disease is an inherited disease caused by point mutation in hemoglobin (β-globin gene). Under oxygen saturation, sickle hemoglobin form polymers, leading to rigid erythrocytes. The transition of the blood vessels is altered and initiated by the adhesion of erythrocytes, neutrophils and endothelial cells. Sickle Hemoglobin (HbS) polymerization is a major cause in red blood cells (RBC), promoting sickling and destruction of RBCs. Isoquercitrin, a medicinal bioactive compound found in various medicinal plants, has multiple health benefits. The present study examines the potential of isoquercitrin as an anti-sickle agent, showing a significant decrease in the rate of polymerization as well as sickling of RBCs. Isoquercitrin-induced graded alteration in absorbance and fluorescence of HbS, confirmed their interaction. A negative value of ΔG° strongly suggests that it is a spontaneous exothermic reaction induced by entropy. Negative ΔH° and positive ΔS° predicted that hydrogen and hydrophobic binding forces interfered with a hydrophobic microenvironment of β6Val leading to polymerization inhibition of HbS. HbS-Isoquercitrin complex exhibits helical structural changes leading to destabilization of the HbS polymer as confirmed by CD spectroscopy. MST and DSC results indicate greater changes in thermophoretic mobility and thermal stability of sickle hemoglobin in the presence of isoquercitrin, respectively. These findings were also supported by molecular simulation studies using DOCK6 and GROMACS. Hence, we can conclude that isoquercitrin interacts with HbS through hydrogen bonding, which leads to polymerization inhibition. Consequently, isoquercitrin could potentially be used as a medication for the treatment of sickle cell disease.Communicated by Ramaswamy H. Sarma 相似文献
152.
Serological diversity within the species Legionella spiritensis 总被引:2,自引:2,他引:0
T. G. Harrison N. A. Saunders Nivedita Doshi R. Wait A. G. Taylor 《Journal of applied microbiology》1988,65(5):425-431
A strain of Legionella isolated from the environment which could not initially be identified was shown by restriction fragment length polymorphisms to be a Legionella spiritensis. This was confirmed by DNA homology studies, cell wall fatty acid composition and isoprenoid quinone analysis. This strain, which is only the second reported representative of the species, was shown to be serologically distinct from the type strain of L. spiritensis and all other serogroups of Legionella. 相似文献
153.
The role of Hsp27 and actin in the regulation of movement in human cancer cells responding to heat shock 总被引:1,自引:0,他引:1
Human heat shock 27-kDa protein 1 (HSPB1)/heat shock protein (Hsp) 27 is a small heat shock protein which is thought to have
several roles within the cell. One of these roles includes regulating actin filament dynamics in cell movement, since Hsp27
has previously been found to inhibit actin polymerization in vitro. In this study, the role of Hsp27 in regulating actin filament
dynamics is further investigated. Hsp27 protein levels were reduced using siRNA in SW480 cells, a human colon cancer cell
line. An in vitro wound closure assay showed that cells with knocked down Hsp27 levels were unable to close wounds, indicating
that this protein is involved in regulating cell motility. Immunoprecipitation pull down assays were done, to observe if and
when Hsp27 and actin are in the same complex within the cell, before and after heat shock. At all time points tested, Hsp27
and actin were present in the same cell lysate fraction. Lastly, indirect immunostaining was done before and after heat shock
to evaluate Hsp27 and actin interaction in cells. Hsp27 and actin showed colocalization before heat shock, little association
3 h after heat shock, and increased association 24 h after heat shock. Cytoprotection was observed as early as 3 h after heat
shock, yet cells were still able to move. These results show that Hsp27 and actin are in the same complex in cells and that
Hsp27 is important for cell motility.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献